FIG. 5.

Steady-state RNA levels transcribed from the UL4-CAT promoter with either wild-type or a site 2 mutation at early times after infection. HFFs were infected with 5 PFU of RVwt-as or RVdlIE86-as per cell. Cytoplasmic RNA was analyzed as described in the legend to Fig. 4. (A) Autoradiogram of RNase protection assay. Lanes: 1, mock infected; 2, RVwt-as at 24 hpi; 3, RVdlIE86-as at 24 hpi; 4 and 5, ³²P-labeled IE1 and actin riboprobes not treated with RNase, respectively. The sizes of the protected RNAs are indicated (in nucleotides [nt]). Lanes 1 to 3, the ³²P-labeled CAT, IE1, and actin riboprobes were added to the reaction mixture. (B) Image acquisition analysis of the CAT signal normalized to actin.