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. 2023 May 22;92:104608. doi: 10.1016/j.ebiom.2023.104608

Fig. 7.

Fig. 7

Covisualising SARS-CoV-2 RNA species and nucleocapsid-immunoreactive signal in alveoli. Confocal images of sections through alveolar parenchyma of COVID 6 and COVID 1. The extracellular matrix in the alveolar walls emits a bright and diffuse green autofluorescent signal. Nucleocapsid-immunoreactive signal is in blue, where it is in green in the related Fig. 8. (a,b) Confocal images of sections of COVID 6. (a) Nucleocapsid-immunoreactive signal is confined to the alveolar walls; a group of nucleocapsid-immunoreactive cells at the center of the image harbours S puncta and orf1ab puncta. (b) A group of nucleocapsid-immunoreactive cells in another region of the same section harbours S puncta and orf1ab puncta. (c,d) Confocal images of sections of COVID 1. (c) Large groups of nucleocapsid-immunoreactive cells and debris fill the alveolar spaces; distinct S puncta and orf1ab puncta are scattered within the nucleocapsid-immunoreactive signal. (d) Groups of cells and debris in the alveolar space contain nucleocapsid-immunoreactive signal and harbour clusters of S puncta and orf1ab puncta. The names of RNAscope probes are in italics and the names of antigens/proteins that are revealed as immunoreactive signal by antibodies are in roman.