Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 13;6:0120. doi: 10.34133/research.0120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Junzhuang Chang et al.

Exclusive Licensee Science and Technology Review Publishing House. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0).

PMC Copyright notice

Fig. 1. — Lymphatic vessels exist deep in the mouse brain. (A) Schematic representation of experimental design of iDISCO+ tissue clearing procedure, immunostaining, and whole-brain imaging by light-sheet microscopy. (B) Light-sheet microscope fluorescent images of LYVE1 immunostaining of cleared whole-mount brain from different views. (C) Examples of optical sections taken from of whole-mount mouse brain from coronal (Y), sagittal (X), horizontal (Z), and orthogonal planes. Insets: Selected areas within the optical sections magnified to show the fluorescent signal of LYVE1 staining. (D) Schematic representation of experimental design of CLARITY tissue clearing procedure, immunostaining, and confocal microscopy imaging of thick brain sections. (E) Confocal images of LYVE1 fluorescent in cleared 1-mm-thick coronal brain section from mouse brain. Selected areas within the section magnified to show the fluorescent signal of LYVE1 staining in different areas. (F) Coimmunostaining of LYVE1 and CD34 in 1-mm coronal section. (G) Coimmunostaining of LYVE1 and CD235a in 16-μm brain section. (H) Same as (F) but coimmunostaining of LYVE1 and CD3. White arrows indicate T cells detected within the deep brain lymphatic vessels. (I) Coimmunostaining of LYVE1 and podoplanin (PDPN) in 16-μm brain section. (J) Same as (I) but coimmunostaining of LYVE1 and VEGFR3. (K) Same as (I) but coimmunostaining of LYVE1 and PROX1. DAPI, 4′,6-diamidino-2-phenylindole.