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. 2023 May 22;18(5):e0285812. doi: 10.1371/journal.pone.0285812

Fig 6. Comparison of human PRMT7-mediated methylation of human H2B (23–37), Xenopus H2B (23–37), and synthetic peptide substrates over time.

Fig 6

The methylation activity over time of GST-HsPRMT7 with human histone H2B (23–37), Xenopus histone H2B (23–37), mono R and RGR peptide substrates was compared using the P81 phosphocellulose paper assay as described in Materials and Methods. Left-hand side: radioactive counts-per-minute of reactions containing 5 μg of GST-HsPRMT7 incubated with 10 μM Hs H2B, Xl H2B (23–37), mono R, or RGR peptide and 0.14 μM [3H]-AdoMet for 0, 15, 30, 60, and 120 min at 25°C. Right-hand side: statistical significance was determined for GST-HsPRMT7 only, Xl H2B (23–37), mono R, and RGR at each time point via Student’s t-test. All reactions indicated were run in triplicate. Hs H2B (23–37), H. sapiens histone H2B (23–37) peptide; Xl H2B (23–37), X. laevis histone H2B (23–37) peptide; mono R, Ac-KKGGRGGGGKKY-amide; RGR, Ac-KKGGRGRGGKKY-amide.