Table 1. Summary of protein and peptide substrates.
| Protein or Peptide Name | Sequence | Theoreticalc,d m.w. (Da) | Experimentald m.w. (Da) | HPLC Puritye |
|---|---|---|---|---|
| H. sapiens H2B | - a | 13,906 a,c | — | — |
| X. laevis H2B | - b | 13,934 b,c | — | — |
| H. sapiens H2B (23–37) | Ac-KKDGKKRKRSRKESY-amide | 1935.25 | 1935.6 | 87.8% |
| X. laevis H2B (23–37) | KKDGKKRRKSRKESY | 1894.20 | 1894.0 | 88.8% |
| Mono R | Ac-KKGGRGGGGKKY-amide | 1234.39 | 1233.0 | 95.5% |
| RGR | Ac-KKGGRGRGGKKY-amide | 1332.56 | 1332.3 | 92.0% |
| KRGR | Ac-KKGKRGRGGKKY-amide | 1403.69 | 1403.2 | 96.0% |
| RKR | Ac-KKGGRKRGGKKY-amide | 1403.69 | 1403.2 | 97.9% |
| RGRK | Ac-KKGGRGRKGKKY-amide | 1403.69 | 1403.2 | 98.1% |
| RER | Ac-KKGGRERGGKKY-amide | 1404.63 | 1404.4 | 97.0% |
a Obtained from UNIPROT accession number P62807.
b Obtained from UNIPROT accession number P02281.
c Theoretical molecular weight using Expasy Compute pI/Mw tool.
d Theoretical/experimental molecular weight determined by GenScript.
e Experimentally determined HPLC purity reported on GenScript peptide analysis certificate.