TABLE 1.
The effect of fusion with human cells on p24CA and infectious virus production by HIV-1-infected rodent cellsa
| Infected cell (× fusion partner) | p24 in supernatant (ng/ml)b | Infectious virus titer (IU/ml)c | Infectivity (IU/pg of p24) |
|---|---|---|---|
| MDTF | 4.1 (1.0)d | 700 (1.0) | 0.17 (1.0) |
| MDTF × MDTF | 3.1 (0.8) | 1,000 (1.4) | 0.31 (1.8) |
| MDTF × HOS | 11.4 (2.8) | 68,000 (97.1) | 5.92 (34.8) |
| Rat2 | 11.2 (1.0) | 4,000 (1.0) | 0.35 (1.0) |
| Rat2 × Rat2 | 10.5 (0.9) | 2,600 (0.7) | 0.24 (0.7) |
| Rat2 × HOS | 19.8 (1.8) | 240,000 (60.0) | 12.12 (34.6) |
a
Supernatant fluid was harvested from HIV-1-infected rodent cells after PEG fusion with homologous rodent cells or with human HOS cells.
b
Viral p24CA antigen was determined by ELISA.
c
Infectious virus titer was determined by using the focal immunoassay as described in Materials and Methods.
d
Numbers in parentheses indicate values relative to unfused cells, which were arbitrarily set at 1.0.