Table 3.
Soil SEED hierarchical microbial (level 4), RefSeq bacterial and eukaryotic expressed genes associated with control and neonicotinoid seed treatment at different times
Soil microbial genes that are significantly differentially expressed (adjusted P<0.05) among different times and between control and neonicotinoid-treated samples in a 2 year soybean/corn rotation in L'Acadie, Quebec, Canada, identified by differential expression analysis of sequence data (DESeq2).
|
Condition |
Variable |
Gene expression |
|||||
|---|---|---|---|---|---|---|---|
|
SEED Hierarchy (Level 4 | Level 1) |
Log2-fold change |
RefSeqBacteria |
Log2-fold change |
RefSeqEukaryotes |
Log2-fold change |
||
|
Only Treatment |
All log2-fold changes |
||||||
|
Treatment |
Control |
− |
− |
− |
− |
− |
− |
|
Neonicotinoid-treated |
− |
− |
− |
− |
− |
− |
|
|
Treatment & Time |
All log2-fold changes |
||||||
|
Treatment in June |
Control |
− |
− |
MULTISPECIES: phosphonate C-P lyase system protein PhnG |
−17.04 |
− |
− |
|
MULTISPECIES: beta-aspartyl-peptidase |
−3.41 |
||||||
|
Neonicotinoid-treated |
− |
− |
− |
− |
− |
− |
|
|
Treatment in September |
Control |
− |
− |
MULTISPECIES: chaperone protein ClpB |
−2.34 |
− |
− |
|
Neonicotinoid-treated |
− |
− |
− |
− |
− |
− |
|
|
Treatment in 2016 |
Control |
− |
−- |
MULTISPECIES: chaperone protein ClpB |
−2.62 |
− |
− |
|
MULTISPECIES: heat-shock protein IbpA |
−2.2 |
||||||
|
Neonicotinoid-treated |
Phycobilisome core-membrane linker polypeptide | Light-harvesting complexes |
3.6 |
Protochlorophyllide oxidoreductase |
2.05 |
− |
− |
|
|
Excinuclease ABC subunit A paralogue in greater Bacteroides group | DNA repair |
0.61 |
||||||
|
Treatment in 2017 |
Control |
Inner membrane protein CreD | Bacteriocins, ribosomally synthesized antibacterial peptides |
−0.85 |
− |
− |
− |
− |
|
Neonicotinoid-treated |
− |
− |
− |
− |
− |
− |
|