Table 1.
Quantification of the major QSSMs in cell-free culture supernatants of P. aeruginosa PAO1
Bacteria were grown aerobically or anaerobically in continuous culture at 37 °C (or 25 °C) at µ=0.05 (slow) or µ=0.31h−1 (fast) in complete or nutrient-limited CDM.
|
Growth condition |
Growth rate (μ) |
OD600 |
C4-HSL (μm) |
3OC12-HSL (μm) |
HHQ (μm) |
PQS (μm) |
HQNO (μm) |
|---|---|---|---|---|---|---|---|
|
Complete |
Slow |
1.13 |
12.8 |
2.04 |
1.2 |
2.16 |
11.3 |
|
Fast |
1.36 |
5.8 |
0.93 |
0.38 |
0.3 |
1.58 |
|
|
Carbon |
Slow |
0.38 |
4.7 |
0.49 |
0.12 |
0.05 |
0.5 |
|
Fast |
0.29 |
2.8 |
0.23 |
<0.01 |
<0.01 |
0.09 |
|
|
Nitrogen |
Slow |
0.21 |
4.2 |
0.25 |
0.12 |
<0.01 |
<0.01 |
|
Fast |
0.20 |
2.4 |
0.17 |
0.04 |
<0.01 |
<0.01 |
|
|
Phosphate |
Slow |
0.66 |
6.7 |
0.51 |
3.52 |
6.00 |
100.7 |
|
Fast |
0.53 |
5.6 |
0.48 |
1.8 |
3.2 |
38.8 |
|
|
Sulfur |
Slow |
0.3 |
1.26 |
0.03 |
2.9 |
3.8 |
122.0 |
|
Fast |
0171 |
0.65 |
0.08 |
0.3 |
0.2 |
4.8 |
|
|
Iron |
Slow |
1.29 |
1.6 |
0.024 |
0.57 |
1.43 |
7.4 |
|
Fast |
1.02 |
0.8 |
0.04 |
0.59 |
1.01 |
1.81 |
|
|
Magnesium |
Slow |
1.39 |
4.8 |
0.21 |
0.43 |
0.52 |
0.52 |
|
Fast |
1.54 |
1.5 |
0.35 |
0.34 |
0.19 |
0.11 |
|
|
Anaerobic |
Slow |
0.18 |
2.12 |
0.24 |
0.13 |
0.26 |
0.45 |
|
Fast |
0.15 |
0.2 |
<0.01 |
0.04 |
<0.01 |
0.10 |
|
|
25 °C |
Slow |
1.02 |
9.33 |
0.42 |
0.03 |
0.06 |
0.77 |
|
Fast |
0.58 |
6.04 |
0.45 |
0.02 |
0.04 |
0.54 |