Table 2.
Quantification of the 3OC12-HSL turnover products 3OC12-HS and TMA in cell free culture supernatants of P. aeruginosa PAO1
Bacteria were grown aerobically or anaerobically in continuous culture at 37 °C (or 25 °C) at µ=0.05 h−1 or µ=0.31h1 in complete or nutrient limited CDM.
|
Growth condition |
Growth rate (μ) |
OD600 |
3OC12-HSL (μM) |
3OC12-HS (μM) |
TMA (μM) |
|---|---|---|---|---|---|
|
Complete |
Slow |
1.13 |
2.04 |
0.19 |
0.07 |
|
Fast |
1.36 |
0.93 |
0.12 |
0.01 |
|
|
Carbon |
Slow |
0.38 |
0.49 |
0.16 |
0.06 |
|
Fast |
0.29 |
0.23 |
0.10 |
0.04 |
|
|
Nitrogen |
Slow |
0.21 |
0.25 |
0.35 |
<0.01 |
|
Fast |
0.20 |
0.17 |
0.38 |
<0.01 |
|
|
Phosphate |
Slow |
0.66 |
0.51 |
0.07 |
<0.01 |
|
Fast |
0.53 |
0.48 |
0.14 |
<0.01 |
|
|
Sulfur |
Slow |
0.30 |
0.03 |
0.29 |
0.45 |
|
Fast |
0.17 |
0.08 |
0.35 |
0.61 |
|
|
Iron |
Slow |
1.29 |
0.02 |
0.35 |
0.16 |
|
Fast |
1.02 |
0.04 |
0.48 |
0.30 |
|
|
Magnesium |
Slow |
1.39 |
0.21 |
0.19 |
0.06 |
|
Fast |
1.54 |
0.35 |
0.19 |
0.04 |
|
|
Anaerobic |
Slow |
0.18 |
0.24 |
nd |
2.4 |
|
Fast |
0.15 |
<0.01 |
nd |
1.75 |
|
|
Temperature |
Slow |
1.02 |
0.42 |
nd |
0.05 |
|
Fast |
0.582 |
0.45 |
nd |
0.05 |
ND, not determined.