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Primer sequences for RT-qPCR
| Primer sequence name | Primer sequence (5′ to3′) |
|---|---|
| ALP (F) | ACCACCACGAGAGTGAACCA |
| ALP (R) | CGTTGTCTGAGTACCAGTCCC |
| Col-1 (F) | ACAGGCATAAAAGGCCCACT |
| Col-1 (R) | GGACTTCCGTAGCCTGGTTT |
| Sp-7 (F) | CCTGCGACTGCCCTAATT |
| Sp-7 (R) | GCGAAGCCTTGCCATACA |
| Runx-2 (F) | ACTTCCTGTGCTCGGTGCT |
| Runx-2 (R) | GACGGTTATGGTCAAGGTGAA |
| GAPDH (F) | GAAAGCCTGCCGGTGACTAA |
| GAPDH (R) | TGGAATTTGCCATGGGTGGA |
