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. Author manuscript; available in PMC: 2024 Jan 20.
Published in final edited form as: J Vis Exp. 2023 Jan 20;(191):10.3791/64308. doi: 10.3791/64308
Name of Material/ Equipment Company Catalog Number Comments/Description
0.45 μm filter Millipore SCHVU05RE
10 mL syringe BD Biosciences 309604
12% polyacrylamide gel with 10 wells Bio-Rad 4561043
12-well plate Corning 07–200-82
18 G needle BD Biosciences 305195
25 G needle BD Biosciences 305122
50 mL tube Fisher Scientific 50–809-218
70 μm cell strainer Corning 431751
150 mm tissue culture dishes Corning 430597
182-cm2 tissue culture flask Genesee Scientific 25–211
Accessory white trans tray Cytiva 29–0834-18
Anti–caspase-1 antibody AdipoGen AG-20B-0042-C100
Anti–caspase-11 antibody Novus Biologicals NB120–10454
Anti–caspase-3 antibody Cell Signaling Technology 9662
Anti–caspase-7 antibody Cell Signaling Technology 9492
Anti–caspase-8 antibody Cell Signaling Technology 4927
Anti–caspase-9 antibody Cell Signaling Technology 9504
Anti–cleaved caspase-3 antibody Cell Signaling Technology 9661
Anti–cleaved caspase-7 antibody Cell Signaling Technology 9491
Anti–cleaved caspase-8 antibody Cell Signaling Technology 8592
Anti-mouse HRP-conjugated secondary antibody Jackson ImmunoResearch Laboratories 315–035-047
Anti-rabbit HRP-conjugated secondary antibody Jackson ImmunoResearch Laboratories 111–035-047
Anti-rat HRP-conjugated secondary antibody Jackson ImmunoResearch Laboratories 112–035-003
Anti–β-Actin antibody (C4) HRP Santa Cruz sc-47778 HRP
ATP InvivoGen tlrl-atpl
BBL Trypticase Soy Broth BD Biosciences 211768
Bead bath Chemglass Life Sciences CLS-4598–009
Biophotometer D30 Eppendorf 6133000010
BME Sigma M6250
Bromophenol blue Sigma BO126
Cell scrapers CellTreat Scientific Products 229315
Chemiluminescence imager (Amersham 600) Cytiva 29083461
CO2 chamber VetEquip 901703
Cuvettes Fisher Scientific 14–955-129
Dissecting scissors Thermo Fisher Scientific 221S
DMEM Thermo Fisher Scientific 11995–073
DTT Sigma 43815
Eelectrophoresis apparatus Bio-Rad 1658004
Ethanol Pharmco 111000200
Fetal bovine serum Biowest S1620
Filter paper Bio-Rad 1703965
Forceps Fisher Scientific 22–327379
Francisella novicida (U112 strain) BEI Resources NR-13
Gel releaser Bio-Rad 1653320
Gentamycin Gibco 15750060
Glycerol Sigma G7893
Glycine Sigma G8898
HCl Sigma H9892
Heat block Fisher Scientific 23–043-160
Herpes simplex virus 1 (HF strain) ATCC VR-260
High glucose DMEM Sigma D6171
Human anti–caspase-1 antibody R&D Systems MAB6215
Human anti–caspase-8 antibody Enzo ALX-804–242
Humidified incubator Thermo Fisher Scientific 51026282
Image analysis software ImageJ v1.53a
IMDM Thermo Fisher Scientific 12440–053
Influenza A virus (A/Puerto Rico/8/34, H1N1 [PR8]) constructed per Hoffmann et al.
L929 cells ATCC CCL-1 cell line for creating L929-conditioned media
L-cysteine Thermo Fisher Scientific BP376–100
Luminata Forte Western HRP substrate Millipore WBLUF0500 standard-sensitivity HRP substrate
MDCK cells ATCC CCL-34 cell line for determining IAV viral titer
Methanol Sigma 322415
Microcentrifuge Thermo Fisher Scientific 75002401
Non-essential amino acids Gibco 11140050
Nonfat dried milk powder Kroger
NP-40 solution Sigma 492016
PBS Thermo Fisher Scientific 10010023
Penicillin and streptomycin Sigma P4333
Petri dish Fisher Scientific 07–202-011
PhosSTOP Roche PHOSS-RO
Power source Bio-Rad 164–5052
Protease inhibitor tablet Sigma S8820
PVDF membrane Millipore IPVH00010
Rocking shaker Labnet S2035-E
SDS Sigma L3771
Sodium chloride Sigma S9888
Sodium deoxycholate Sigma 30970
Sodium hydroxide Sigma 72068
Sodium pyruvate Gibco 11360–070
Square Petri dish Fisher Scientific FB0875711A
Stripping buffer Thermo Fisher Scientific 21059
Super Signal Femto HRP substrate Thermo Fisher Scientific 34580 high-sensitivity HRP substrate
Tabletop centrifuge Thermo Fisher Scientific 75004524
Trans-Blot semi-dry system Bio-Rad 170–3940
Tris Sigma TRIS-RO
Tween 20 Sigma P1379
Ultrapure lipopolysaccharide (LPS) from E. coli 0111:B4 InvivoGen tlrl-3pelps
Vero cells ATCC CCL-81 cell line for determining HSV1 viral titer