| 0.45 μm filter |
Millipore |
SCHVU05RE |
|
| 10 mL syringe |
BD Biosciences |
309604 |
|
| 12% polyacrylamide gel with 10 wells |
Bio-Rad |
4561043 |
|
| 12-well plate |
Corning |
07–200-82 |
|
| 18 G needle |
BD Biosciences |
305195 |
|
| 25 G needle |
BD Biosciences |
305122 |
|
| 50 mL tube |
Fisher Scientific |
50–809-218 |
|
| 70 μm cell strainer |
Corning |
431751 |
|
| 150 mm tissue culture dishes |
Corning |
430597 |
|
| 182-cm2 tissue culture flask |
Genesee Scientific |
25–211 |
|
| Accessory white trans tray |
Cytiva |
29–0834-18 |
|
| Anti–caspase-1 antibody |
AdipoGen |
AG-20B-0042-C100 |
|
| Anti–caspase-11 antibody |
Novus Biologicals |
NB120–10454 |
|
| Anti–caspase-3 antibody |
Cell Signaling Technology |
9662 |
|
| Anti–caspase-7 antibody |
Cell Signaling Technology |
9492 |
|
| Anti–caspase-8 antibody |
Cell Signaling Technology |
4927 |
|
| Anti–caspase-9 antibody |
Cell Signaling Technology |
9504 |
|
| Anti–cleaved caspase-3 antibody |
Cell Signaling Technology |
9661 |
|
| Anti–cleaved caspase-7 antibody |
Cell Signaling Technology |
9491 |
|
| Anti–cleaved caspase-8 antibody |
Cell Signaling Technology |
8592 |
|
| Anti-mouse HRP-conjugated secondary antibody |
Jackson ImmunoResearch Laboratories |
315–035-047 |
|
| Anti-rabbit HRP-conjugated secondary antibody |
Jackson ImmunoResearch Laboratories |
111–035-047 |
|
| Anti-rat HRP-conjugated secondary antibody |
Jackson ImmunoResearch Laboratories |
112–035-003 |
|
| Anti–β-Actin antibody (C4) HRP |
Santa Cruz |
sc-47778 HRP |
|
| ATP |
InvivoGen |
tlrl-atpl |
|
| BBL Trypticase Soy Broth |
BD Biosciences |
211768 |
|
| Bead bath |
Chemglass Life Sciences |
CLS-4598–009 |
|
| Biophotometer D30 |
Eppendorf |
6133000010 |
|
| BME |
Sigma |
M6250 |
|
| Bromophenol blue |
Sigma |
BO126 |
|
| Cell scrapers |
CellTreat Scientific Products |
229315 |
|
| Chemiluminescence imager (Amersham 600) |
Cytiva |
29083461 |
|
| CO2 chamber |
VetEquip |
901703 |
|
| Cuvettes |
Fisher Scientific |
14–955-129 |
|
| Dissecting scissors |
Thermo Fisher Scientific |
221S |
|
| DMEM |
Thermo Fisher Scientific |
11995–073 |
|
| DTT |
Sigma |
43815 |
|
| Eelectrophoresis apparatus |
Bio-Rad |
1658004 |
|
| Ethanol |
Pharmco |
111000200 |
|
| Fetal bovine serum |
Biowest |
S1620 |
|
| Filter paper |
Bio-Rad |
1703965 |
|
| Forceps |
Fisher Scientific |
22–327379 |
|
|
Francisella novicida (U112 strain) |
BEI Resources |
NR-13 |
|
| Gel releaser |
Bio-Rad |
1653320 |
|
| Gentamycin |
Gibco |
15750060 |
|
| Glycerol |
Sigma |
G7893 |
|
| Glycine |
Sigma |
G8898 |
|
| HCl |
Sigma |
H9892 |
|
| Heat block |
Fisher Scientific |
23–043-160 |
|
| Herpes simplex virus 1 (HF strain) |
ATCC |
VR-260 |
|
| High glucose DMEM |
Sigma |
D6171 |
|
| Human anti–caspase-1 antibody |
R&D Systems |
MAB6215 |
|
| Human anti–caspase-8 antibody |
Enzo |
ALX-804–242 |
|
| Humidified incubator |
Thermo Fisher Scientific |
51026282 |
|
| Image analysis software |
ImageJ |
v1.53a |
|
| IMDM |
Thermo Fisher Scientific |
12440–053 |
|
| Influenza A virus (A/Puerto Rico/8/34, H1N1 [PR8]) |
constructed per Hoffmann et al. |
|
|
| L929 cells |
ATCC |
CCL-1 |
cell line for creating L929-conditioned media |
| L-cysteine |
Thermo Fisher Scientific |
BP376–100 |
|
| Luminata Forte Western HRP substrate |
Millipore |
WBLUF0500 |
standard-sensitivity HRP substrate |
| MDCK cells |
ATCC |
CCL-34 |
cell line for determining IAV viral titer |
| Methanol |
Sigma |
322415 |
|
| Microcentrifuge |
Thermo Fisher Scientific |
75002401 |
|
| Non-essential amino acids |
Gibco |
11140050 |
|
| Nonfat dried milk powder |
Kroger |
|
|
| NP-40 solution |
Sigma |
492016 |
|
| PBS |
Thermo Fisher Scientific |
10010023 |
|
| Penicillin and streptomycin |
Sigma |
P4333 |
|
| Petri dish |
Fisher Scientific |
07–202-011 |
|
| PhosSTOP |
Roche |
PHOSS-RO |
|
| Power source |
Bio-Rad |
164–5052 |
|
| Protease inhibitor tablet |
Sigma |
S8820 |
|
| PVDF membrane |
Millipore |
IPVH00010 |
|
| Rocking shaker |
Labnet |
S2035-E |
|
| SDS |
Sigma |
L3771 |
|
| Sodium chloride |
Sigma |
S9888 |
|
| Sodium deoxycholate |
Sigma |
30970 |
|
| Sodium hydroxide |
Sigma |
72068 |
|
| Sodium pyruvate |
Gibco |
11360–070 |
|
| Square Petri dish |
Fisher Scientific |
FB0875711A |
|
| Stripping buffer |
Thermo Fisher Scientific |
21059 |
|
| Super Signal Femto HRP substrate |
Thermo Fisher Scientific |
34580 |
high-sensitivity HRP substrate |
| Tabletop centrifuge |
Thermo Fisher Scientific |
75004524 |
|
| Trans-Blot semi-dry system |
Bio-Rad |
170–3940 |
|
| Tris |
Sigma |
TRIS-RO |
|
| Tween 20 |
Sigma |
P1379 |
|
| Ultrapure lipopolysaccharide (LPS) from E. coli 0111:B4 |
InvivoGen |
tlrl-3pelps |
|
| Vero cells |
ATCC |
CCL-81 |
cell line for determining HSV1 viral titer |