Table 1.

Primer sequences used for malaria detection (target gene: SSU 18S rRNA of circum-sporozoite protein, CSP)

Plasmodium genus nest 1 (1.6–1.7 kb)	rPLU1	5′ TCAAAGATTAAGCCATGCAAGTGA 3′
	rPLU5	5′ CCTGTTGTTGCCTTAAACTTC 3′
Plasmodium genus nest 2 (235 bp)	rPLU3	5′ TTTTTATAAGGATAACTACGGAAAAGCTGT 3′
	rPLU4	5′ TACCCGTCATAGCCATGTTAGGCCAATACC 3′
P. falciparum (206 bp)	rFal1	5′ TTAAACTGGTTTGGGAAAACCAAATATATT 3′
	rFal2	5′ ACACAATGAACTCAATCATGACTACCCGTC 3′
P. vivax (121 bp)	rVIV1	5′ CGCTTCTAGCTTAATCCACATAACTGATAC 3’
	rVIV2	5′ ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA 3′
P. ovale (226 bp)	rOVA1	5′ ATCTCTTTTGCTATTTTTTAGTATTGGAGA 3′
	rOVA2	5′ GGAAAAGGACACATTAATTGTATCCTAGTG 3′
P. malariae (145 bp)	rMAL1	5′ ATAACATAGTTGTACGTTAAGAATAACCGC 3′
	rMAL2	5′ AAAATTCCCATGCATAAAAAATTATACAAA 3’
P. knowlesi (200 bp)	Pkr140(F)	5′ CAGAGATCCGTTCTCATGATTTCCATGG3′
	Pkr140(R)	5′ CTRAACACCTCATGTCGTGGTAG3′