FIG. 1.

(A) Genetic map of AAV. Transcripts and protein-encoding regions for the three AAV promoters are shown. Promoters and intron donor and acceptor sites are mapped with their AAV nucleotide designations. Antisense AAV RNA probes used in our assays are positioned relative to the AAV genome and are described in Materials and Methods. (B) Schematic representation of probe fragments protected by AAV transcripts. Individual fragments are diagramed in their approximate relative positions when separated on a 6% denaturing polyacrylamide gel. U, unspliced; s, sliced.