Appendix 1—table 2. Parameters used in the mathematical model.
| Parameter | Notation | Value | Units | Notes |
|---|---|---|---|---|
| Receptor–ligand binding | k 1 | 0.03 | nM–1 s–1 | Schoeberl et al., 2002 |
| Ligand dissociating from L-EGFR (β = 1 corresponds to EGF) | k 2 | β*6.6e-3 | s–1 | Macdonald and Pike, 2008 |
| Ligand dissociating from L-EGFR:EGFR (β = 1 corresponds to EGF) | k 3 | β*5.7e-3 | s–1 | Macdonald and Pike, 2008 |
| Ligand dissociating from L-EGFR:L-EGFR | k 4 | 0.087 | s–1 | Macdonald and Pike, 2008 |
| Receptor dimerization and activation | k 5 | 1e-5 | nM–1 s–1 | Estimated |
| EGFR:EGFR dissociation | k 6 | 5e-3; variable values in Figure 3—figure supplement 2. | s–1 | Estimated |
| L-EGFR:EGFR dissociation (γ = 1 corresponds to EGF) | k 7 | γ*1e-4 | s–1 | Estimated |
| ZtSH2 binding to receptor | k 8 | 5 | nM–1 s–1 | Kd from Ottinger et al., 1998; kinetics set to be ~10 s based on our experimental measurements of ZtSH2 translocation |
| ZtSH2 dissociating from receptor | k 9 | 16.67 | s–1 | Kd from Ottinger et al., 1998; kinetics set to be ~10 s based on our experimental measurements of ZtSH2 translocation |
| Scaling parameter for ligand–receptor binding | β | 1 for EGF; 50 for low-affinity ligands; variable values in Figure 3C | Unitless | Freed et al., 2017 |
| Scaling parameter for dimerization of ligand-bound receptors | γ | 1 for EGF; 100 for low-affinity ligands; variable values in Figure 3C | Unitless |
Freed et al., 2017; Hu et al., 2022 |