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In vivo aortic stiffness is higher with aging in both male and female C57BL/6N mice, but only in male B6D2F1 mice. Aortic pulse wave velocity (PWV) in male and female, young (6–7 mo) and old (28–31 mo) B6D2F1 (A) and young (6–7 mo) and old (27–29 mo) C57BL/6N (B) mice. Data are represented as means ± SD; n = 6–15/group. Statistics are two-way mixed (age × sex) ANOVA with Šidák’s post hoc test. *P < 0.05; ***P < 0.001; ****P < 0.0001.
