Figure 3.

Aortic wall structural and extracellular matrix remodeling in male and female mice with aging. In male and female, young (6–7 mo) and old (27–29 mo) C57BL/6N mice, aortic diameter (A) and aortic intima-media thickness (IMT; B), measured in 7 μm segments of thoracic aorta; representative images of aortic diameter and IMT (C); media cell count using DAPI staining (D); and adventitial abundance of collagen-1 (E), measured via immunofluorescence in 7 μm segments of thoracic aorta; representative images of DAPI (blue) and collagen-1 (red) (F). Data are represented as means ± SD; n = 5–13/group. Statistics are two-way mixed (age × sex) ANOVA with Šidák’s post hoc test. *P < 0.05; ***P < 0.001; ****P < 0.0001.