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. 2023 Apr 13;324(5):E461–E475. doi: 10.1152/ajpendo.00059.2023

Figure 2.

Figure 2.

Orchiectomized (ORX) mice exposed to high-fat, high-sucrose diet (HFHS) have increased astrocytosis but not microgliosis in the mediobasal hypothalamus. A and B: representative images showing glial fibrillary acidic protein (GFAP) immunoreactivity in the mediobasal hypothalamus (MBH) of sham-operated (Sham)-HFHS (A) and ORX-HFHS (B) mice. 3V, third ventricle. Scale bar, 500 µm. C: quantification of GFAP-positive area percentage in the MBH from 6 sections per animal. D and E: representative images showing Iba1 immunoreactivity in the MBH of Sham-HFHS (D) and ORX-HFHS (E) mice. F: quantification of Iba1-positive area percentage in the MBH from 6 sections per animal. Data are presented as means ± SE of 4 mice per group.