Table 1.
Tooth organoid medium (TOM) composition
| Product | Concentration | Supplier | Catalog number |
|---|---|---|---|
| Serum-free defined medium (SFDM) | For composition, see Table S1 | ||
| A83-01 | 0.5 μM | Sigma-Aldrich | SML0788 |
| B27 (without vitamin A) | 1X | Gibco | 12587-010 |
| Cholera toxin | 100 ng/mL | Sigma-Aldrich | C8052-.5mg |
| FGF2 (=basic FGF) | 20 ng/mL | R&D Systems | 234-FSE |
| FGF8 | 200 ng/mL | Peprotech | AF-100-25 |
| FGF10 | 100 ng/mL | Peprotech | 100-26 |
| L-glutamine | 2 mM | Gibco | 25030081 |
| IGF1 | 100 ng/mL | Peprotech | 100-11 |
| N2 | 1X | Gibco | 17502-048 |
| N-acetyl L-cysteine | 1.25 mM | Sigma-Aldrich | A7250 |
| Nicotinamide | 10 mM | Sigma-Aldrich | N0636 |
| Noggin | 100 ng/mL | Peprotech | 120-10C |
| RSPO1 | 200 ng/mL | Peprotech | 120-38 |
| SB202190 | 10 μM | Biotechne (Tocris) | 1264 |
| SHH | 100 ng/mL | R&D Systems | 464-SH |
| WNT3A | 200 ng/mL | R&D Systems | 5036-WN |
| EGFa | 20 ng/ML | R&D Systems | 236-EG |
a
For all results from Figure 3 onward, TOM was by default supplemented with EGF.