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. 2023 May 9;18(5):1138–1154. doi: 10.1016/j.stemcr.2023.04.004

Figure 1.

Figure 1

Subretinally transplanted human cells migrate from, or remain in, the subretinal space

(A) IHC staining of human nuclear antigen (HNA) showed migratory (arrows) and non-migratory (empty arrow) human cells in the mouse retina. Transplanted photoreceptors were identified by the expression of CRX:tdTomato reporter.

(B and C) Migratory cells were detected overlying the graft (radial migration) and beyond the graft edge (tangential migration).

(D) A non-transplanted mouse eye showed negative staining for HNA and CRX:tdTomato.

(E) Relative abundance of migratory human cells in different recipient retinal laminae.

(F) Migrating distance of human cell nuclei from the graft edge in different retinal laminae.

(G) Migratory Ku80+ human cells found in the regions flanking the optic nerve (peripapillary migration). White arrows showed representative migratory human cells.

Data were collected from 5 eyes. Statistical data were presented as mean ± SD. Yellow lines in (B), (C), (D), and (G) denote the boundaries of the recipient retina; green lines in (G) denote the optic nerve.