Subretinally transplanted human cells migrate from, or remain in, the subretinal space
(A) IHC staining of human nuclear antigen (HNA) showed migratory (arrows) and non-migratory (empty arrow) human cells in the mouse retina. Transplanted photoreceptors were identified by the expression of CRX:tdTomato reporter.
(B and C) Migratory cells were detected overlying the graft (radial migration) and beyond the graft edge (tangential migration).
(D) A non-transplanted mouse eye showed negative staining for HNA and CRX:tdTomato.
(E) Relative abundance of migratory human cells in different recipient retinal laminae.
(F) Migrating distance of human cell nuclei from the graft edge in different retinal laminae.
(G) Migratory Ku80+ human cells found in the regions flanking the optic nerve (peripapillary migration). White arrows showed representative migratory human cells.
Data were collected from 5 eyes. Statistical data were presented as mean ± SD. Yellow lines in (B), (C), (D), and (G) denote the boundaries of the recipient retina; green lines in (G) denote the optic nerve.