Figure 3.

Donor-derived migratory cells include astrocytes and brain/spinal cord-like neural precursors

(A) Astrocytes and BSL cells show the highest migration score among the cell types identified in transplanted retinal organoids (n = 3 eyes).

(B) RNAScope showed migratory cells expressing markers of astrocytes (PAX2, HES6) and BSL cells (ASCL1, HOXC8, NKX2-2, and ARX).

(C) Relative abundance of migratory astrocytes and BSL cells in different recipients’ retinal laminae.

(D) Quantification of migratory astrocytes and BSL cells in migratory human cells (n = 3 or 4 eyes).

(E and F) RNAScope staining (E) and quantification (F) of non-migratory astrocytes and BSL cells in the subretinal space (SRS) (n = 4 eyes).

(G) Migratory cells negatively expressed markers of RGC (RBPMS, NeuN), amacrine cells (NeuN), horizontal cells (calbindin), rod bipolar cells (PKCα), cone bipolar cells (SCGN), microglia (IBA1), and macrophage (CD68). KU80- and HNA-labeled human cells.

Statistical data were presented as mean ± SD.