Figure 4.

Actively proliferating cells are rare among migratory and non-migratory donor cells

(A) IHC staining showed that Ki-67⁺ proliferating cells were rare in cultured organoids (n = 4 in one batch) and significantly less in transplanted organoids (n = 3 eyes).

(B) Ki-67⁺ cells were rare among non-migratory and migratory cells in transplanted retinal organoids (n = 5 eyes).

(C) Relative abundance of migratory Ki-67⁺ cells in different retinal laminae.

(D) ScRNA-seq analysis showed the highest proliferation score in Müller glia, retinal progenitor cells (RPCs), astrocytes, and BSL cells among the identified cell types in transplanted retinal organoids (n = 3 eyes).

(E) Histological assay showed sparse Ki-67⁺ cells in migratory astrocytes (PAX2⁺), BSL cells (ASCL1⁺), and non-migratory RPCs (VSX2⁺) and BSL cells (HOXC8⁺) (n = 4 eyes).

Statistical data were presented as mean ± SD.