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. 2022 Oct 26;72(2):164–172. doi: 10.1538/expanim.22-0104

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©2023 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 2. — Deficiency of IRG1 and itaconate exacerbated histological changes, inflammation, and alveolar exudation. (A, B) The expression of IRG1 in lung tissue was analyzed by Western blot. (C) The levels of itaconate in lung tissue were detected by UPLC-MS/MS. (D) The plasma levels of IL-6 were determined by ELISA. (E, F) Histological score was determined by hematoxylin and eosin (HE) staining (400×). (G) Comparison of Evans Blue leakage in lung tissue. (H) Comparison of protein concentration in BALF. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. ALI, acute lung injury; IRG1, aconitate decarboxylase; UPLC-MS/MS, ultrahigh-performance liquid chromatography-tandem mass spectroscopy; IL-6, interleukin 6; BALF, bronchoalveolar lavage fluid.