Figure S4.

Mapping of antibody-binding regions and calculated distances between CGN and CGNL1 and ZO proteins. (Related to Fig. 5.) (A–C and G) IB analysis (top panels) and Ponceau-S labeling (bottom panels) of bacterially expressed GST-fusion protein fragments of CGN (A and C), NM2B (B), and CGNL1 (G) using the indicated antibodies, to map the antibody epitopes. (D–F and H–J) IF microscopy analysis of the localization of endogenous CGN in MDCK WT cells (D) and CGNL1 in EpH4 WT cells (H). High magnification panels correspond to highlighted white box in low magnification micrograph; scale bars = 10 μm (low magnification) and 1 μm (high magnification) for (D) and (H). (E, F, I, and J) Linescan analysis of signal distribution (E and I) and box plots of distances of CGN signal from ZO-2 signal (F) and CGNL1 signal from ZO-1 signal (J). For E, F, I, and J, n = 24 from two independent experiments, and data in quantifications are represented as mean ± SD. Source data are available for this figure: SourceData FS4.