Fig. 3.

ORF3a-mediated ferroptosis is regulated by NRF2-ARE pathway. (A) Tet-on inducible NCI–H1299 cells were treated with or without doxycycline for 24h, followed by treatment with RSL3 (0.5 μM) for 12h, mRNA levels of indicated genes were quantified via qRT-PCR analysis. Quantitations are normalized to actin. Data show mean ± SEM from three independent experiments. (B&C) HEK 293T cells were transfected with indicated plasmids along with NQO-1-luc plasmid and pRL-TK for 36 h and then luciferase activity was determined by Dual Luciferase Reporter Assay. Data show mean ± SEM from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***p ≤ 0.001, n.s. = not significant.