Fig. 7. MANF downregulates BATF2 by inhibiting CHOP expression.

a A schematic diagram of mouse BATF2 promoter and its truncates cloned to the luciferase reporter. The luciferase reporter was co-transfected into 293T cells along or with MANF plasmid. The cells were challenged with LPS at 24 h after transfection, and then the luciferase activity was measured 24 h after LPS stimulation. b Effect of mutation of CHOP binding sites on BATF2 transcription. The mutants were co-transfected into 293T cells with MANF plasmid. Twenty-four hours after transfection, the cells were treated with LPS for 24 h, then the luciferase activity was measured. c The protein levels of CHOP, BATF2, and IL-12 were detected in colon tissues from the mice treated with DSS. d The quantitative data in panel c. All data are expressed as mean ± SEM from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.