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. 2023 May 22;13:8213. doi: 10.1038/s41598-023-34943-w

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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ACCT vs Ilastik vs CellProfiler vs Basic Fiji on images of Iba-1 positive microglia. The performance of ACCT classifier 10 versus Ilastik, CellProfiler, and basic use of Fiji tools. The audit set is a selection of 10 images, which is equal to the size of the validation image set, chosen from the experimental dataset and evenly distributed between experimental groups. Error bars represent standard error of the mean on calculated performance statistics from each image, where the statistic itself is calculated from total cells in the dataset. The parameters used by ACCT in this analysis were: a 0.5 threshold, 50 minimum pixel size, 1,000 maximum pixel size for objects.