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. 2023 May 22;14(5):337. doi: 10.1038/s41419-023-05857-2

Fig. 5. Multiplex IF/ISH analysis of TMAs enable improved characterization and spatial information relative to the miR-21-5p repression by CDK2AP1.

Fig. 5

A Representative images of CDK2AP1-positive (left) and -negative (right) TMA tumor cores featuring low and high expression of miR-21-5p, respectively. Next to the microscope images, digital reconstructions with tumor cells as red dots, and stromal cells as gray dots are depicted. In the lower panels, higher magnifications of the corresponding inlets are shown. B CDK2AP1 and miR-21-5p quantification of expression in the tumor and stroma components of the cores in (A). Asterisks denote the significance of the P values by one-sample t test (****P < 0.0001). C. Visualization of intra-tumor heterogeneity of CDK2AP1 and miR-21-5p expression levels in a TMA core and the digital reconstructions of the same microscope image. Opposite expression gradients of CDK2AP1 protein and miR-21-5p can be observed. In the lower panels, higher magnifications of the corresponding inlets are shown. D Box plot analysis of CDK2AP1 expression across miR-21-5p high and low groups (P = 0.006; paired t test).