Fig. 3. IFT20 and TSG101 interact and synergize with each other.

A Schematic linear diagram of TSG101. B Schematic linear diagram of IFT20. C Yeast cells transformed with the IFT20 plasmid and prey plasmids containing potential IFT20-binding partners were cultured on nonselective (+histidine and adenosine) or selective SD media (–histidine and adenosine). The growth of yeast on selective media indicates the presence of protein‒protein interactions. D Yeast cells transformed with the TSG101 plasmid and prey plasmids containing potential IFT20-binding partners were cultured on nonselective (+histidine and adenosine) or selective SD medium (–histidine and adenosine). The growth of yeast on selective medium indicated the presence of protein‒protein interactions. E Coimmunoprecipitation of IFT20 and TSG101 from lysates of HEK293 T cells overexpressing the indicated proteins. F Coimmunoprecipitation of endogenous IFT20 and TSG101 proteins in Jurkat cell lysates. Jurkat cells were activated with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies for 24 h. In E–F, the data represent more than three independent experiments. UEV ubiquitin E2 variant, CC coiled coil, IB immunoblot, IP immunoprecipitation