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. 2023 Apr 7;20(5):525–539. doi: 10.1038/s41423-023-01008-x

Fig. 4. IFT20 acts as a critical regulator of c-SMAC formation.

Fig. 4

A–F Naïve CD4+ T cells from WT and IFT20-KO mice were enriched by magnetic beads and activated with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies for 8 h (n = 5). A Representative immunofluorescence confocal microscopy images of unstimulated and stimulated CD4+ T cells from WT and IFT20-KO mice stained for CD3 (blue), IFT20 (red), TSG101 (green), and DAPI (light blue). Using ZEN 3.2 software, 3D images were obtained. Scale bar, 5 μm. CD3 intensities (B) and CD3 cluster ratios (C) were calculated with ZEN 3.2 software. D Representative immunofluorescence confocal microscopic images of unstimulated and stimulated CD4+ T cells from WT and IFT20-KO mice stained for CD28 (blue), IFT20 (red), TSG101 (green), and DAPI (light blue). 3D images were obtained with ZEN 3.2 software. Scale bar, 5 μm. CD28 intensities (E) and CD28 cluster ratios (F) were calculated with ZEN 3.2 software. The images in (A) and (D) represent five independent experiments. In B–C and E–F, the data represent more than three independent experiments. The data are shown as the mean ± SEM. Statistical significance was calculated by unpaired Student’s t test. ***P < 0.001