Fig. 7. TSG101 deficiency reduced CD4⁺ T cell activation.

A–D CD4⁺ T cells from the spleens of TSG101-KO and WT mice were enriched by magnetic beads and stimulated with 1 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies for 8 h. A Representative flow cytometry plots showing the expression of CD44 and CD62L in unstimulated and stimulated CD4⁺ T cells from WT and TSG101-KO mice. B The frequencies of CD44^-CD62L⁺, CD44⁺CD62L^-, and CD44⁺CD62L⁺ T cells were measured by flow cytometry (n = 8). C Representative flow cytometry plots showing the expression of CD25 and CD69 in unstimulated and stimulated CD4⁺ T cells from WT and IFT20-KO mice. D The frequencies of CD25⁺(CD25⁺CD69⁺ and CD25⁺CD69^-), CD69⁺(CD25⁺CD69⁺ and CD25^-CD69⁺), and CD25⁺CD69⁺ T cells were measured by flow cytometry (n = 8). In A–D, the data represent more than three independent experiments. CD4⁺ T cells were obtained by negative selection using a CD4⁺ T cell enrichment kit. The data are expressed as the mean ± SEM. Significance was calculated using an unpaired Student’s t test. ***P < 0.001