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. 2023 May 23;9:173. doi: 10.1038/s41420-023-01470-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — (a) Heat map of GO selection of autophagy-specific proteins from entire proteome mass spectrometry data. qRT-PCR analysis of autophagy markers LC3 and P63 in individuals with DSD (b, c) and TGCT (d, e). Immunoblotting for LC3 and P62 proteins for controls (f), Swyer and Swyer-GCT (g), CAIS and CAIS-GCT (h), Turner (i), Klinefelter (j). Quantification of the ratio of LC3-II to LC3-I (k) and P62 relative protein levels normalized to α-actinin (l) in DSD-individuals. Immunoblotting of autophagy markers for TGCT group (m) and quantification (n, o). Unpaired t test was used for statistical analysis, p values are indicated.