Table 1.

Current studies using CRISPR-based screens in hPSC-derived cells

Year	Stem cell type	Differentiated cell type	CRISPR type	Screening strategy	Phenotype	Library size	Reference
2018	hiPSC	hepatocyte-like cell	CRISPRn	FACS	hepatocytic differentiation	genome-wide	Li et al.15
2019	hiPSC & hESC	neural progenitor	CRISPRn	survival	cytotoxicity of Zika virus infection	genome-wide	Li et al.16
2019	hiPSC	glutamatergic neuron	CRISPRi	survival	neuronal survival	CRISPRi-v2 H1 library (∼2,000 genes encoding kinases, phosphatases, and drug targets)	Tian et al.17
				single-cell transcriptomics	transcriptome changes in response to gene knockdown	27 hit genes
				imaging	neurite morphology	23 hit genes
2020	hESC	cerebral organoid	CRISPRn	proliferation	cerebral organoid growth	172 microcephaly candidate genes	Esk et al.18
2020	hESC	cardiac mesoderm and progenitor	CRISPRn	FACS	formation of cardiac mesoderm and progenitors (MSP1 and ISL1 staining)	∼6,000 genes	Xu et al.19
2021	hiPSC	glutamatergic neuron	CRISPRi/a	survival	neuronal survival under normal and oxidative stress conditions	genome-wide (hCRISPRi/a-v2 library)	Tian et al.20
				FACS	levels of ROS and lipid peroxidation	genome-wide & a focused library against 730 hit genes
				single-cell transcriptomics	transcriptome changes in response to gene knockdown	184 hit genes for CRISPRi and 100 hit genes for CRISPRa
2021	hiPSC	cardiomyocyte	CRISPRn	survival	doxorubicin-induced cardiotoxicity	genome-wide	Sapp et al.21
2022	hiPSC	neural stem cell	CRISPRi	proliferation; FACS	cell proliferation during neuronal induction; neural differentiation (PAX6 staining)	genome-wide libraries against coding and lncRNA genes	Wu et al.22
				single-cell transcriptomics screens	transcriptome changes in response to gene knockdown	240 targets (120 coding genes and 120 lncRNA genes)
2022	hiPSC	human subpallial organoid	CRISPRn	FACS	interneuron differentiation	425 neurodevelopmental disorder related genes	Meng et al.23
		human forebrain assembloid			interneuron migration
2022	hiPSC	cortical neuron	CRISPRn	survival	cytotoxicity of PR20 dipeptide repeats	kinome-wide (736 kinases)	Guo et al.24
2022	hiPSC	astrocyte	CRISPRi	FACS	inflammatory reactivity of astrocytes (synaptosome phagocytosis; cell-surface VCAM1 levels)	human transcription factors and druggable genome (～4,000 targets)	Leng et al.25
				single-cell transcriptomics	transcriptome changes in response to gene knockdown	30 hit genes
2022	hiPSC	microglia	CRISPRi/a	survival/proliferation	microglia survival and proliferation	CRISPRi-v2 H1 library (∼2,000 genes encoding kinases, phosphatases, and drug targets)	Dräger et al.26
				FACS	microglia activation (CD38 staining)
					microglia phagocytosis of pHrodo-Red-labeled synaptosomes
				single-cell transcriptomics	transcriptome changes in response to gene knockdown	39 hit genes
2022	hiPSC	kidney organoid	CRISPRn	proliferation	kidney development and cell proliferation	genome-wide	Ungricht et al.27

This table summarizes current studies using CRISPR-based screens in hPSC-derived cells. CRISPRn, CRISPR knockout; CRISPRi, CRISPR interference; CRISPRa, CRISPR activation; hPSC, human pluripotent stem cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; FACS, fluorescence-activated cell sorting.