Figure 3 .

Concentration dependence data for cytochrome C. (A) The chromatograms for I_0.02 and the scaled Abs₂₈₀ are plotted, respectively. The residuals between these two are shown at the bottom. (B) Chromatogram for I(0) and the R_g distribution obtained by the Guinier analysis. (C) The scattering profiles. The scaled scattering profile for peak (black) and the form factors, A(Q)s calculated with rank=1 (red) and rank=2 (blue) are displayed, respectively (left y-axis). The scattering profile for the concentration-dependent component B(Q) is reconstructed with rank=2 and displayed in gray (right y-axis). (D) The comparison of A(Q) (top) and B(Q) (bottom) obtained from SEC-SAXS (black) and conventional (gray) measurements. (E) Scaled A(Q) (black) and B(Q) (gray) reconstructed with rank=2. The scaling factor for A(Q) was calculated based on minimizing the value calculated with Equation (6). The difference between B(Q) and the scaled A(Q) is shown at the bottom (black dotted line). (F) The concentration dependence for R_g from the Guinier analysis in both the original scattering profiles (gray) and the reconstructed scattering profiles calculated with rank=1 (black) and rank=2 (red).