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. 2023 Jan 7;20(1):e200001. doi: 10.2142/biophysico.bppb-v20.0001

Figure 5 .

Figure 5 

Peak decomposition analysis for thyroglobulin. (A) The mapping result for the chromatograms for I0.02 and the scaled Abs280. The residuals between these two are plotted at the bottom. (B) The forward scattering I(0) (line) (left y-axis), I(0)/concentration (closed circle), and Rg (open circle) (right y-axis, respectively) are plotted against the frame number. (C) Decomposition result of the chromatogram for I0.02 (black solid line) using EGH. The dotted lines and the gray solid line correspond to the decomposed components and the sum of the decomposed components, respectively. The residuals between the sum of the decomposed components and the experimental curve are shown at the bottom. Scattering profiles for the first peak (D) and the second peak (E) are reconstructed using rank=1 (black line) and rank=2 (gray line), respectively. The differences between B(Q) and the scaled A(Q) for rank=2 are calculated based on Equation (6) and displayed at the bottom, respectively.

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