TABLE 2.
Effects of antisense DNA oligonucleotide targeting the viral DLS region on viral replication
| Treatment | Titera | Reduction in titer (fold)b | Relative fold reductionb in:
|
Reduction in packaged RNAsc | |
|---|---|---|---|---|---|
| Genomic RNAs | Virion RNAs | ||||
| No oligonucleotide | 3.70 (0.35) | NAd | 1.00 | 1.00 | NA |
| antiPBS | 0.51 (0.05) | 6.00 | 0.95 (0.07) | 0.66 (0.08) | 1.44 |
| antiPal | 0.61 (0.01) | 5.00 | 0.83 (0.25) | 0.27 (0.02) | 3.10 |
| antiG5 | 0.50 (0.09) | 7.70 | 0.81 (0.13) | 0.26 (0.04) | 3.12 |
| antiG3 | 1.30 (0.07) | 2.60 | 1.00 (0.01) | 0.58 (0.01) | 1.72 |
| antiCon1 | 2.80 (0.63) | 1.00 | 0.80 (0.22) | 0.55 (0.05) | 1.46 |
| antiCon2 | 3.20 (0.64) | 1.20 | 0.95 (0.07) | 0.77 (0.05) | 1.23 |
| antiNeo | 0.75 (0.14) | 3.00 | 0.88 (0.18) | 0.91 (0.16) | 0.91 |
| revNeo | 2.90 (0.14) | 1.20 | 0.93 (0.11) | 0.97 (0.04) | 1.02 |
| Standard | 2.30 (0.78) | 2.00 | 0.83 (0.04) | 0.75 (0.11) | 1.11 |
a
Titers (105 CFU per milliliter) of the MuSV vector from a representative experiment. The numbers in parentheses represent standard deviations.
b
Mean values (fold reduction) normalized to the values for the untreated samples. Data are taken from at least two independent assays. The numbers in parentheses represent standard deviations.
c
Relative fold reduction of packaged RNAs = relative fold reduction of genomic RNAs/relative fold reduction of virion RNAs.
d
NA, not applicable.