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. 2023 May 22;14:2937. doi: 10.1038/s41467-023-38539-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Comparison of particle picking accuracy in the raw tomogram and in the REST-restored tomogram. The offsets of each picked particle in the X-, Y-, and Z-directions to the ground truth either in the raw tomogram or in the REST-restored tomogram are shown. B Comparison of the CC values calculated between the picked particles and templates in the raw tomograms and in the REST restored tomograms, respectively. C REST-restored particles present roughly accurate orientations. Left: Direct reconstruction result of the 892 raw particles using the orientation calculated from REST-restored density, Middle: The averaged map calculated from the same raw particle set using the traditional STA strategy. Right: EMD-11999 filtered to 15 Å for comparison. D Top: Raw subtomograms (contrast enhanced by NAD filtering). Middle: Subtomograms restored by REST. Bottom: Remapped averaging density map in the position of raw particle corresponding to the orientations determined by STA.