Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 22;14:2900. doi: 10.1038/s41467-023-38624-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Plasma levels of the neutrophil chemoattractant CXCL7 are reduced in Cxcl7ko (Cxcl7^-/-) mice. CXCL5 and CXCL4 (encoded by adjacent genes) are also reduced whereas CXCL1 is not affected. The graphs display the mean ± SD with n = 4 (CXCL7) and n = 5 (CXCL1, CXCL4, CXCL5) biologically independent samples from n = 4 and n = 5 independent mice, respectively; *P < 0.05 and **P < 0.01 (unpaired two-tailed t test). b, c H&E staining of TA muscles from control and Cxcl7ko mice at day 1, 7, and 14 from cardiotoxin (CTX) injection, and uninjured. Immune infiltration at day 1 after CTX-mediated injury is reduced in the muscles from Cxcl7ko mice. Ultrastructural defects can be seen at day 14 post-injury in muscles from Cxcl7ko versus control mice. d Immunostaining of muscles from control and Cxcl7ko mice for neutrophil markers, i.e., Ly6G (purple). Myofiber boundaries are identified with immunostaining for anti-Laminin antibodies (green) whereas nuclei are stained with DAPI (blue). e Intramuscular neutrophil infiltration at day 1 from injury is significantly reduced in Cxcl7ko mice. Similar results are found with both Ly6G and MMP9 immunostaining. The graphs display the mean ±SD with n = 5 biologically independent samples for each group and condition from n = 5 independent mice; **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-way ANOVA with Tukey post hoc test); ^&P < 0.05 and ^&&&P < 0.001 (two-way ANOVA with Sidak post hoc test) refer to the comparison of CTX-injured WT and Cxcl7ko at a given timepoint of regeneration. f Normally, platelets (green) are found in association with neutrophils (red) in injured muscles. Although lack of CXCL7 decreases neutrophil recruitment, the area of platelet thrombi that is found in injured muscles of WT and Cxcl7ko mice is similar, indicating that defective neutrophil recruitment does not result from lower platelet number or aggregation in Cxcl7ko mice, consistent with previous studies that have found that platelet numbers and hemostatic functions are not impaired in these mice⁹⁹. The graph displays the mean ±SD with n = 5 biologically independent samples for each group and condition from n = 5 independent mice; ***P < 0.001 (two-way ANOVA with Tukey post hoc test). Source data are provided in the Source Data file.