Table 1.

Differential features of the new isolated strains and closely related species.

Characteristics	1	2	3	4a	5b	6c	7d	8e	9f	10g	11h
Cell size	0.7–1.0 × 2.8-3.0	0.7–1.0 × 3.0–5.0	0.5–0.7 × 3.0–5.0	0.3–0.5 × 2.0–6.0	0.5–0.7 × 2.0–4.0	0.5–0.7 × 2.0–4.0	0.3–0.5 × 4.0–6.0	0.4–0.6 × 5.0–8.1	0.7–0.8 × 1.2–2.9	0.7–1.2 × 3.5–5.0	0.2–0.6 × 1.4–5.6
Colony pigmentation	White	White	White	White	White	Milk	White	Cream	White	Cream	White
Motility	+	+	+	NA	+	+	+	+	+	+	−
NaCl range (%, w/v)	0.5–17	0.5–20	NA	1–17	0–14	0–14	0.5–20	0–10	1–20	0.5–20	0–12
NaCl optimum (%, w/v)	7	2	NA	5–10	5–8	5–8	10	4	10	7–10	0–8
pH range	6–8	4–9	NA	4–9	5–12	6–9	6.5–9	6–9	6–10	5.5–9	6–9
pH optimum	6	5	NA	7	7	7	7	7	8	7	6–8.5
Temperature range (° C)	11–45	10–45	NA	15–45	10–40	10–40	20–40	25–45	10–45	15–45	20–50
Temperature optimum (° C)	37	37	NA	25–30	30	30	35	37	37	35	35
Anaerobic growth	+	+	+	−	−	−	−	−	+	NA	−
Aesculin hydrolysis	+	−	−	+*	+	+	+*	+*	+	+	NA
Urease	−	−	−	+*	−	+	+*	+*	NA	+	−
Nitrite reduction	+	−	−	+*	−	+	−*	−*	NA	+	NA
G + C content (mol%, genome)	38.0	38.0	38.1	36.7	35.9	36.0	36.8	36.9**	35.7	37.4	38.5

1. Strain 3ASR75-54^T; 2. Strain 3ASR75-11^T; 3. Strain 3ASR75-286; 4. Aquibacillus albus YIM 93624^T (G + C content from A. albus DSM 23711^T); 5. Aquibacillus halophilus B6B^T; 6. Aquibacillus kalidii HU2P27^T; 7. Aquibacillus koreensis BH30097^T (G + C content from A. koreensis JCM 12387^T); 8. Aquibacillus salifodinae WSY08-1^T; 9. Aquibacillus saliphilus KHM2^T; 10. Aquibacillus sediminis BH258^T; 11. Radiobacillus deserti TKL69^T. NA, not available. *, data from this study. **, determined by HPLC.

^aZhang et al. (2012);

^bAmoozegar et al. (2014);

^cWang et al. (2021);

^dLee et al. (2006);

^eZhang et al. (2015);

^fCho and Whang (2022);

^gLee and Whang (2019);

^hLi et al. (2020).