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. 2023 Apr 20;3(5):100303. doi: 10.1016/j.xgen.2023.100303

Figure 3.

Figure 3

blots validate and quantify exRBPs in cell-conditioned media

(A) Diagram of serum-free cell culture for protein extraction from paired cell lysate and concentrated conditioned media and following RBP detection by western blot. Created with BioRender.

(B) Western blots performed in triplicate on 293T and hMSC cell lysate and conditioned media to quantitate ratios in cell-conditioned media vs. cell lysate.

(C) Ratio of detected signal between conditioned media and corresponding cell lysate blots for 293T and hMSCs. A value greater than 1 indicates stronger detection of the RBP in the extracellular space, while a value below 1 shows stronger detection intracellularly. “nD” indicates lack of intracellular detection in hMSCs. Data are represented as mean +SD.