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. 2022 Dec 24;4(3):100506. doi: 10.1016/j.xplc.2022.100506

Figure 2.

Figure 2

Amino acid sequence alignment of NbUGT72AY1 and StUGT72AY2 from Nicotiana benthamiana and Solanum tuberosum, respectively, and HDX results.

The catalytically active H18, activating D118, plant secondary product glycosyltransferase (PSPG) consensus sequence position 352–395, and G461/S462/S463 motif are highlighted by black bars and arrows; mutations ① N27D, ② IF/VI double mutant, ③ chimeric mutant, and ④ H/Y mutant are highlighted by red bars and arrows. Amino acids of NbUGT72AY1 that showed reduced hydrogen/deuterium exchange in HDX experiments in the presence of scopoletin are framed with blue boxes (Supplemental Figure S9A).