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. 2022 Dec 24;4(3):100506. doi: 10.1016/j.xplc.2022.100506

Figure 5.

Figure 5

Kinetic parameters of NbUGT72AY1, StUGT72AY2, and mutant enzymes using scopoletin and sinapyl aldehyde as substrates.

(A) NbUGT72AY1 and its mutants were used to glucosylate scopoletin.

(B) StUGT72AY2 and its mutants were used to glucosylate scopoletin.

(C) NbUGT72AY1 and its mutants were used to glucosylate sinapyl aldehyde.

(D) StUGT72AY2 and its mutants were used to glucosylate sinapyl aldehyde. Experimental data were obtained by UDP-Glo glycosyltransferase assay and fitted to the partial uncompetitive inhibition model (Equation 4). The amino acid sequence information of the mutants is shown in Figure 2. Data represent mean ± SD of n = 3 technical replicates.