Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 22;14:2939. doi: 10.1038/s41467-023-38583-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Left: experimental strategy for labeling projections of Glut⁺ thoraco-lumbar neurons bilaterally. Right: transverse sections showing transfected spinal neurons. Scale bars, 500 µm. b Distribution of eYFP⁺ transfected cells in different thoraco-lumbar segments. Bar-graphs are the mean number ± SD per hemi-section, and gray circles are mean values of n individual mice. c Left: transverse section at the preBötC level showing eYFP⁺ spinal projections. Scale bar, 1 mm. Right: magnification of the preBötC area containing SST⁺ cells. Note the absence of spinal projections. Scale bar, 100 µm. Representative of n = 3 mice. d Transverse section at the level of the pF showing eYFP⁺ spinal projections. Scale bar, 1 mm. Right: magnification showing abundant eYFP⁺ spinal projections in the pF area, ventrally and ventro-medially to the 7 N motoneurons. e Mean density ± SD across n mice of eYFP⁺ fluorescent pixels in preBötC and pF hemi-sections. Gray circles are the mean values of individual mice. ** denotes p = 0.0039 (Wilcoxon matched-pairs tests; 9 sections per area). f Left: delineation of rostro-caudal (top to bottom) and medio-lateral (orange and blue) subdivisions of the pF. Right: distribution of fluorescent pixels in the pF subdivisions. Bars are means ± SD across n mice and gray circles are mean values of individual mice. g Left: experimental strategy for labeling synaptic boutons of Glut⁺ thoraco-lumbar neurons. Injections were done bilaterally. Right: transverse section showing putative synaptic boutons in the pF area. Scale bars, 250 (top) and 50 (bottom) µm. Representative of n = 3 mice. h Left: experimental strategy for labeling pF neurons contacted by spinal neurons through anterograde transsynaptic transfer of an AAV1-Syn-Cre in adult wild-type mice. Injections were done bilaterally. Right: transverse section showing eYFP⁺ neurons in the pF area demonstrating that spinal ascending projections make synaptic contacts with pF neurons. Representative of n = 3 mice. Scale bar, 200 µm. i Quantification of transsynaptically labeled neurons in the pF subdivisions defined in f. See “Methods” for details. For all graphs, source data are provided as a Source data file. See also Fig. S8.