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. 2023 Jan 10;44(6):1191–1205. doi: 10.1038/s41401-022-01044-9

Fig. 4. The inhibition of UGCG impairs lysosomal homeostasis and promotes lipid droplet accumulation.

Fig. 4

a The expression of LC3B, LAMP1 in LX2 cells were detected by immunofluorescence staining. b Lysosomal pH was detected by LysoSensor probe. c The maturation of Cathepsin L, after treat with PDMP was verified by Western blot. d Fluorescence images of cells were measured by antibody against Cathepsin L. e After PDMP treatment, the distribution of TFEB in cytoplasm and nucleus was detected by Western blot. f Intracellular differential lipid metabolites after PDMP treatment were used for KEGG signaling pathway analysis. g Schematic diagram of lysosomal imbalance caused by GlcCer defect. h The effect of PDMP on intracellular lipid droplets was observed by oil red staining. i The relative mRNA expression of RARA and RARB was detected by qPCR. The experiments were repeated three times, and statistical significance was determined by a t-test. **P < 0.01.