Skip to main content
. 2023 May 22;13:8276. doi: 10.1038/s41598-023-35281-7

Figure 1.

Figure 1

Generation of mice with inducible genetic deletion of LepR in endothelial cells. (A) Tamoxifen-induced, Cre recombinase-mediated gene excision of leptin receptors (∆LepR) in brain, visceral adipose tissue (VAT), subcutaneous adipose tissue (SCAT), small intestine and lung tissue biopsies. The full-length images of the gels from which the areas of interest showing bands were cropped are shown in the Supplementary information file. Quantitative real-time PCR analysis of LepR, long (B,D,F) and short (C,E,G) isoform, mRNA levels in endothelial cells isolated from brain (B,C; n = 5–7 mice per group), VAT (D,E; n = 6 mice per group) or lung (F,G; n = 6–8 mice per group) of End.LepR-WT and End.LepR-KO mice. Data were analyzed using Student’s t test (C–G) or Mann–Whitney test (B), depending on the presence of normal distribution. *p < 0.05, **p < 0.01 and ****p < 0.001. (H) Representative fluorescence microscopy images of LepR expression (magenta) on CD31-immunopositive endothelial cells (green) in brain, VAT and lungs of End.LepR-WT and End.LepR-KO mice. Cell nuclei were visualized using DAPI (blue). Size bars represent 20 µm.