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. 2023 May 22;13:8276. doi: 10.1038/s41598-023-35281-7

Figure 7.

Figure 7

Energy metabolism and glycolytic enzyme or mitochondrial marker expression in primary lung endothelial cells. Representative examples of the extracellular acidification rate (ECAR; A) and the oxygen consumption rate (OCR; B) in live primary endothelial cells isolated from female End.LepR-WT (n = 7) and End.LepR-KO (n = 7) mice fed standard diet. Data were analyzed using Two-Way ANOVA, Sidak’s multiple comparison tests. *p < 0.05 and **p < 0.01 vs. End.LepR-WT mice at the same time point. Non-significant differences are not shown. The summary of findings from n = 4 independent experiments measuring the ECAR (C) or OCR (D) to determine glycolysis or mitochondrial respiration in End.LepR-WT and End.LepR-KO mice fed standard diet (SD) or high-fat diet (HFD). Results were normalized to the number of cells and are expressed as -fold change vs. End.LepR-WT mice. Data were compared using One-Way ANOVA, Sidak’s multiple comparison tests. *p < 0.05, **p < 0.01 and ****p < 0.0001 vs. End.LepR-WT mice. Quantitative real-time PCR analysis of Glut1 (E), Idh (F) and Sirt3 (G) in primary lung endothelial cells from female End.LepR-WT and End.LepR-KO mice (n = 10 per group). Data were compared using Student’s t test. Ns non-significant.