TABLE 1.
EBNA2 latent gene expression in latently infected naive and IgD− B cells from tonsilsa
| Tonsil no. | No. of infected cells/107 B cells
|
EBNA2 expression
|
||
|---|---|---|---|---|
| IgD+ | IgD− | IgD+ | IgD− | |
| 1 | >400 | >800 | − | − |
| 2 | >200 | >550 | + | − |
| 3 | 200 | 2,300 | + | − |
| 4 | 200 | 400 | + | − |
| 5 | 200 | 333 | + | − |
| 6 | 200 | 200 | + | − |
| 7 | 150 | >800 | + | − |
| 8 | 140 | 80 | + | − |
| 9 | 140 | 70 | + | − |
| 10 | 100 | 1,000 | + | − |
| 11 | 75 | >400 | + | − |
| 12 | 75 | 330 | − | − |
| 13 | 75 | 200 | − | − |
| 14 | 50 | >400 | + | − |
| 15 | 40 | 2,600 | + | − |
| 16 | 40 | 100 | + | − |
a
Frequencies are expressed as the absolute number of virus-infected cells per 107 B cells in the population. The frequencies were measured as detailed previously (12). Briefly, purified cell populations were serially diluted, and multiple aliquots were made of each diluted sample. DNA PCR was then performed on each aliquot, the fraction of negative aliquots for each dilution was estimated, and Poisson statistics were used to calculate the frequencies. Since the DNA PCR will detect a single genome of viral DNA, this assay provides an absolute quantitative measure of the frequency of infected cells.