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Determination of Δorf02889 physiological phenotypes. (A–D) The swimming and swarming motility, siderophore production (CAS assay), and biofilm formation of WT and mutant strains. (E) Survival rates of zebrafish after challenged with Δorf02889. (F) Zebrafish inoculated with Δorf02889 and PBS were challenged with A. hydrophila LP-2 for 7 days. Data represent the mean ± SEM of n ≥ 2 independent replicates. *P < 0.05; **P < 0.01.
