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. 2023 May 9;14:1191209. doi: 10.3389/fimmu.2023.1191209

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Copyright © 2023 Zhang, Sun, Srinivasan, Lin, Gong and Lin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The binding assay of orf02889 with the promoters of four candidate genes. (A–E) ChIP-PCR analysis of the binding of orf02889 and the promoter fragments of corC, orf0906, orf01286, and orf04042. The A. hydrophila chromatin was immunoprecipitated without or with anti-His antibody (Input or IP: His) for PCR with the target primer pairs, respectively. The promoter fragments of candidate genes are marked as P_{gene name}; the orf02889 complementary strain was marked as “+”; Δorf02889 carrying empty vector was marked as “-”.