Table 1.
The zebrafish immune response after vaccination with Δorf02889 and WT strain by qPCR, respectively.
| Gene category | Gene name | Δorf02889/WT |
|---|---|---|
| Pro- and anti-inflammatory cytokines | il-1β | 2.69 ± 0.11*** |
| tnf-α | 0.23 ± 0.01*** | |
| tnf-β1 | 0.05 ± 0.01*** | |
| il-6 | 1.65 ± 0.10*** | |
| il-10 | 2.31 ± 0.16*** | |
| Chemokines | cxcl-18b | 1.01 ± 0.04 |
| ccl-34α.4 | 1.96 ± 0.28** | |
| cxcl-8α | 1.85 ± 0.19*** | |
| Anti-bacterial peptides/enzymes | defβl-1 | 2.01 ± 0.06*** |
| lyz-c | 2.14 ± 0.07*** | |
| Antioxidant | cat | 1.85 ± 0.09*** |
| sod-1 | 1.79 ± 0.07*** | |
| prdx-4 | 1.06 ± 0.07 | |
| gstp-1 | 2.12 ± 0.05*** | |
| txndr-1 | 1.88 ± 0.09** |
Values are the mean ± standard deviation of three independent trials. Significant differences between each mutant strain are indicated by asterisks. **p < 0.01**; ***P < 0.001.