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. 2022 Dec 30;4(3):100514. doi: 10.1016/j.xplc.2022.100514

Figure 3.

Figure 3

ERF115 is needed for root growth recovery in the TGRooZ.

(A) Confocal images of meristems of 22SR, 32SR, and 32TGRooZ pERF115:NSL-GUS/GFP. (Left) GFP-tagged expression in green. (Right) GFP overlapped with PI-stained roots. Scale bar, 50 μm.

(B) Confocal images of PI-stained root meristems of 22SR, 32SR, and 32TGRooZ wild type (WT), erf115 mutant, ERF115 over-expressing (ERF115OE), or ERF115SDRX seedlings. White arrowheads indicate the QC, and arrows indicate the end of the meristem. Scale bar, 100 μm.

(C) Quantification of root meristem size (n > 12), root length, number of LRs, and lateral root density of 22SR, 32SR, or 32TGRooZ WT, erf115 mutant, ERF115OE, or ERF115SDRX seedlings at 6 days after transfer to different temperatures (n ≥ 12). Significance was analyzed by ANOVA and Tukey’s honestly significant difference (HSD) post-test. p < 0.05. Different letters indicate significant differences.